Browsing by Author "Rainsford, Philip Ben"

Now showing items 1-2 of 2

    • Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis 

      Rainsford, Philip Ben; Rylandsholm, Fredrik G.; Jakubec, Martin; Silk, Mitchell; Juskewitz, Eric; Ericson, Johanna Ulrica; Svendsen, John Sigurd Mjøen; Engh, Richard Alan; Isaksson, Johan Mattias (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-08-03)
      One strategy to combat antimicrobial resistance is the discovery of new classes of antibiotics. Most antibiotics will at some point interact with the bacterial membrane to either interfere with its integrity or to cross it. Reliable and efficient tools for determining the dissociation constant for membrane binding (K<sub><i>D</sub></i>) and the partitioning coefficient between the aqueous- and ...

    • WIND-PVPA: Water/Ion NMR Detected PVPA to assess lipid barrier integrity in vitro through quantification of passive water- and ion transport 

      Rainsford, Philip Ben; Sarre, Birta R.; Falavigna, Margherita; Brandsdal, Bjørn Olav; Flaten, Gøril Eide; Jakubec, Martin; Isaksson, Johan Mattias (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-22)
      Water/Ion NMR Detected – Phospholipid Vesicle Permeability Assay (WIND-PVPA), is presented as a novel, straightforward and automatable method to assess lipid barrier integrity in vitro. The apparent permeability constants of water- and ions across the PVPA barriers are determined in a one-pot experiment under the influence of membrane-active guest molecules. NMR spectroscopy is used to quantify ...